Using TCSPC-FLIM to measure FRET provides a quantifiable and highly informative approach to study interactions within live cells. This technique can be performed within ESRIC on the Leica SP5 SMD gSTED microscope equiped with a TCSPC Module and picosecond event timer and single photon avalanche detectors (SPADs).

Single-Photon Counting Modules are self-contained modules that meet the low- light-level luminescence, TCSPC, particle sizing and Quantum Communications.

The HydraHarp 400 is a high-end, easy to use, plug and play Time Interval Analyzer (TIA) and Time-Correlated Single Photon Counting (TCSPC) system with scalability for multiple channels (covered by patent DE 10 2008 004 549). The HARPIA-TF is a time-resolved fluorescence measurement module that combines fluorescence upconversion and TCSPC techniques. In fluorescence upconversion, the signal from the sample is mixed in a nonlinear crystal with a gating femtosecond pulse to achieve high temporal resolution, which is limited by the duration of the gate and pump pulses. TCSPC Module.

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The HydraHarp 400 is a high-end, easy to use, plug and play Time Interval Analyzer (TIA) and Time-Correlated Single Photon Counting (TCSPC) system with scalability for multiple channels (covered by patent DE 10 2008 004 549). The SPC-130-EMN is a small size and low-cost time-correlated single photon counting ( TCSPC) module (patented) with high-time resolution for standard fluorescence lifetime applications and for applications requiring a large number of parallel TCSPC channels. The board offers a PCI interface and can be easily installed in nearly all PCs. The TCC2 is a unique electronics module for the measurement of kinetics via Time- Correlated Single Photon Counting (TCSPC) and Multi-Channel Scaling (MCS), and allows three sources and three detectors to be permanently connected. In TCSPC mode, kinetics from a few picoseconds can be measured using a high repetition rate source. TCSPC is a sensitive technique for measuring fluorescence decays on nanosecond time scale and longer. The principle of TCSPC is based on the precise registration of the arrival time of fluorescence photons from a sample. A fluorescence decay curve is constructed from the TCSPC measurement, this curve is used to extract the fluorescence lifetime.

Using TCSPC-FLIM to measure FRET provides a quantifiable and highly informative approach to study interactions within live cells. This technique can be performed within ESRIC on the Leica SP5 SMD gSTED microscope equiped with a TCSPC Module and picosecond event timer and single photon avalanche detectors (SPADs).

Oxford Scholarly Editions Online - Subscription to Shakespeare Module , Ketvirtis​: K4, TCSPC detektorius infraraudonajam spektriniam intervalui , Ketvirtis: II 

TCSPC Imaging, Multiscaler Imaging and FCS Module. High-end TCSPC module. Time channel width down to 813 fs; Electrical Time Resolution < 3 ps RMS ( 6.5 ps FWHM ) 10 MHz saturated count rate; Memory buffer size: 8 million photons; High-speed interface for laser scanning microscopes; Fluorescence lifetime imaging; Phosphorescence lifetime imaging; FCS in combination with fluorescence lifetime All TCSPC modules provided by PicoQuant expect standard TTL pulses for the external markers. This means 2-5 V for a logic “high”-level and 0-0.8 V for a logic “low”-level.

of the measurement that allows the build-up of a TCSPC photon. histogram (i.e. a photon by a lock-in detection software module LOCK-IN toolkit for. LabVIEW 

The HARPIA-TF module supports Becker&Hickl TCSPC devices and detectors. a TCSPC module. To transform the pulse polarity and the pulse amplitude into the standard input range of the TCSPC module a 20 dB attenuator and a passive pulse inverter were inserted in the signal line. The timing reference pulses at the stop input of the TCSPC module come directly from the laser. ID Quantique’s quantum solutions From individual components to fully integrated solutions for practical, real-world industrial applications, ID Quantique’s customers benefit from a wide range of quantum technologies. Integrated, photonics-based systems design – OTDR, LiDAR, TCSPC , spectroscopy Our multidisciplinary project team includes Physicists and Engineers with skills and TCSPC_UROP. Time Correlated Single Photon Counting Module.

chisurf.experiments.experiment module The bh TCSPC products are complemented by bh picosecond diode lasers, detector modules, multi-spectral detector assemblies, and experiment control modules. Based on these components Becker & Hickl supply their own confocal fluorescence lifetime laser scanning microscope and FLIM upgrade kits for laser scanning microscopes of various manufacturers. functionalities of several electronic devices into a single and flexible one: time-tagger, TCSPC module, delay generator, digital conditional filters and counters. A comprehensive, affordable, versatile and scalable series of instruments composed of 3 different main versions: tcspc每个通道内也有接收上升沿信号的死时间，我们用的国产的tcspc死时间小于10ns ； 另外TCSPC还有时间分辨率 ，理想情况下采集时间是连续的，现实情况是仪器的采集时间都是离散的 [13] （可以看作数学上实数和有理数的区别），即 存在一个最小采集时间间隔 ，称之为时间分辨率。 The bh HPM-100 module combines a Hamamatsu R10467-40 GaAsP hybrid PMT tube with the preamplifier and the generators for the PMT operating voltages in one compact housing. The principle of the hybrid PMT in combination with the GaAsP cathode of the R10467-40 yields excellent timing resolution, a clean TCSPC instrument response function, high detection quantum efficiency, and extremely low The module can be operated also in free-running mode, with the SPAD always ON. The main feature of the module is the possibility to increase the Dynamic Range of TCSPC systems thanks to a fast OFF-ON transition.
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In fluorescence upconversion, the signal from the sample is mixed in a nonlinear crystal with a gating femtosecond pulse to achieve high temporal resolution, which is limited by the duration of the gate pulse and is in the range of 250 fs. SPC-150N TCSPC FLIM Module The SPC-150N time correlated single photon counting (TCSPC) fluorescence lifetime imaging (FLIM) module from Becker and Hickl (B&H) is a TCSPC imaging and fluorescence correlation spectroscopy (FCS) module for laser scanning microscopes. Adhering to the standards set by the ORPHEUS product line, each module is contained in a single monolithic aluminium body ensuring excellent optical stability and minimal optical path lengths.

Has anyone once bought one of these products, and tell the TCSPC (time-domain). FastFLIM (proprietary technology) is the new digital frequency domain (DFD) approach to FLIM measurements.
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NIREOS is collaborating with the major TCSPC module providers and the software is compatible with most of the available TCSPC modules on the market. Contact us to discuss how GEMINI could enrich your time-resolved applications, adding the spectral resolution to your experiments!

It allows multi-pulse time-resolved spectroscopic techniques, in which the ongoing pump-probe photodynamics are perturbed by a delayed third pulse. The principle our TCSPC Lifetime Imaging module is shown in fig. 2.

TauFit is the central TCSPC module of PAM, dedicated to the analysis of fluorescence intensity decays and time-resolved anisotropy. The following analysis methods are available: Tail fitting of fluorescence decays and time-resolved anisotropy; Reconvolution fitting of fluorescence decays using: Single, bi- or triexponential model

TCSPC modules can be achieved in combination with fs laser sources and an ultrafast detector such as a MCP-PMT. This  the timing drift for an SPC-140 TCSPC module and a number of commonly used Time-correlated single photon counting (TCSPC) is based on the detection of  Moreover, bh is supplier of TCSPC modules for commercial time-domain optical tomography instruments.

The module contains all electronic components constant fraction discriminators (CFDs), time-to-amplitude converter (TAC), analog-to-digital converter (ADC), multichannel analyzer (MCA timers) on board required for TCSPC. Using TCSPC-FLIM to measure FRET provides a quantifiable and highly informative approach to study interactions within live cells. This technique can be performed within ESRIC on the Leica SP5 SMD gSTED microscope equiped with a TCSPC Module and picosecond event timer and single photon avalanche detectors (SPADs). All hardware modules relevant to the SymPhoTime 64 (SPT64) are correctly installed in the PC. THese modules could be Laser Drivers, Piezo Controllers, TCSPC modules, PCAN controller card, Framegrabber card etc.